茵陈蒿拮抗A FB1 诱发基因突变和小鼠骨髓细胞遗传损伤的实验研究

doi:10.3969/j.issn.1004-616x.1998.01.011

癌变·畸变·突变 ›› 1998, Vol. 10 ›› Issue (1): 35-038.doi: 10.3969/j.issn.1004-616x.1998.01.011

茵陈蒿拮抗A FB1 诱发基因突变和小鼠骨髓细胞遗传损伤的实验研究

陈少华1 　洪振丰2 　罗仁夏3 　林　丽4 　刘凌冰4 　陈紫榕1

1中国人民解放军第476 医院　福州　350002 　2福建省中医学院　3南京军区福州高等医学专科学校　4福建医科大学

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1998-01-30 发布日期:1998-01-30

THE EXPERIMENTAL STUDIES OF ARTEMISIA CAPILLARIS THUMB TO RESTRICT THE MUTAGENICITY AND GENETIC DAMAGES OF THE BONE MARROW CELLS IN MICE INDUCED BY AFLATOXIN B1

Chen Shaohua , Hong Zhenfeng , Luo Renxia , et al

The 476 Hospital of PL A , Fuz hou 　35002

Received:1900-01-01 Revised:1900-01-01 Online:1998-01-30 Published:1998-01-30

摘要/Abstract

摘要： 茵陈蒿是常用的保肝中药,具有提高机体免疫功能和抗御肿瘤的作用。本研究利用茵陈蒿可能具有抗突变作用,对强致癌剂A FB1 进行A mes 试验、小鼠骨髓细胞的遗传毒理学实验,以评价茵陈蒿的抗突变能力。结果表明11 茵陈蒿A mes 试验为阴性。在A FB1 (5μg 皿) 培养基中加入011 ml 浓度分别为01125、0125、015 、1 、2 g ml 的茵陈蒿煎剂, TA 98 和TA 100 的回变菌落抑制率达2219 - 54136 ,差异极其显著( P 0101) 。21 茵陈蒿煎剂对AFB1 诱发的小鼠骨髓嗜多染红细胞微核率、染色体畸变率和姊妹染色单体交换率的增高有明显的拮抗作用( P 0. 01) ,且具有明显的剂量———反应关系。本结果提示茵陈蒿可能对癌症预防与治疗有积极的意义。

关键词: 茵陈蒿, A FB1, 微核, 染色体畸变, 姊妹染色单体交换, A mes 试验

Abstract: Objective To inhibit the mutagenicity and genetic damages of mouse marrow cells induced by Aflatoxin B1 (AFB1) , Artemisia capillaris Thumb (ACT) had been used to evaluate the possibility of anti-mutagenicity. Methods The test s of Ames test , micronucleus , chromasome aberration and sister chromatid exchanges of mouse bone marrow cells were used to detect the mutagenicity and anti2mutagenicity of ACT and AFB1 . Result s As expected , the mutagenicity of ACT was negative. Put 0. 1 ml ACT ext ract (the concernt ration was 0. 125 , 0. 25 , 0. 5 , 1. 0 and 2. 0 g/ ml) to culture medium of AFB1 (5μg/ plate) , the inhibition rates of TA98 and TA100 were 22. 9 - 54. 36 , the difference was very remarkable ( ( P < 0. 01) . The inhibition effects of ACT to micronuclei , chromosome aberration and sister chromatid exchanges indued by AFB1 were very remarkble too , and the relationship of dose2reaction was very evident . Conclusion These results showed that ACT had very st rong inhibition to the mutagenicity and genetic damages induced by AFB1 and might have the function to repair the DNA damages , which suggested that ACT would have greater meaning to prevent or t reat cancers.

Key words: Artemisia capillaris Thumb, Aflatoxin B1, Micronucleus, Chromasome abbrration, Sister chromatd exchanges, Ames test

陈少华　洪振丰　罗仁夏　林　丽　刘凌冰　陈紫榕. 茵陈蒿拮抗A FB1 诱发基因突变和小鼠骨髓细胞遗传损伤的实验研究[J]. 癌变·畸变·突变, 1998, 10(1): 35-038.

Chen Shaohua , Hong Zhenfeng , Luo Renxia , et al. THE EXPERIMENTAL STUDIES OF ARTEMISIA CAPILLARIS THUMB TO RESTRICT THE MUTAGENICITY AND GENETIC DAMAGES OF THE BONE MARROW CELLS IN MICE INDUCED BY AFLATOXIN B1[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 1998, 10(1): 35-038.

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茵陈蒿拮抗A FB1 诱发基因突变和小鼠骨髓细胞遗传损伤的实验研究

THE EXPERIMENTAL STUDIES OF ARTEMISIA CAPILLARIS THUMB TO RESTRICT THE MUTAGENICITY AND GENETIC DAMAGES OF THE BONE MARROW CELLS IN MICE INDUCED BY AFLATOXIN B1

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